Mass Spectrometry and Molecular Analysis

Basic scientific skills | Seminar (SE) | Coursecode: 220574102

Literature databases and effective literature search

2.00

ECTS

1.00

SWS

Search strategies for literature and other databases (introduction to relevant databases, selection of search keys, systematic approach, primary sources, scientific relevance, search documentation ); practical approach models for efficient and effective literature management, reference management software.

Basic scientific skills | Seminar (SE) | Coursecode: 220574101

Team management and interaction

1.00

ECTS

0.50

SWS

Development of attitudes and strategies that aid in increasing team performance and individual satisfaction and reduce conflict when collaborating in a team. The students get to know how to use digital media when working in a team and how to use professionally digital resources of FH JOANNEUM (audio/video conferencing, resource sharing, the LMS moodle, VPN access to resources of the university intranet), data protection and legal issues (FH JOANNEUM data protection guide).

Chromatographic and mass spectrometric applications I | Lecture (VO) | Coursecode: 220574106

Chromatographic and spectrometric methods

2.00

ECTS

1.50

SWS

Theory about chromatographic separation with focus on liquid and gas chromatography (including introduction with thin layer chromatography), Liquid chromatography (instrumentation, important parameters, Van-Deemter-Equation, stationary and mobile phases, reversed-phase , normal-phase, and hydrophilic interaction chromatography, chiral, affinity and gel-permeation chromatography, theory of isocratic and gradient separation, detectors), Gas chromatography (instrumentation, important parameters, Raoult`s law, columns, carrier gas, retention mechanisms, detectors, theorie of temperatur-dependant separation of volatile and semi-volatile molecules)

Chromatographic and mass spectrometric applications I | Lecture/Practical (IL) | Coursecode: 220574107

Chromatographic and spectrometric techniques I

2.00

ECTS

2.00

SWS

(Preparation of analysis plan, standards and dilution series, measurement of standard samples using chromatographic and spectrometric techniques, Preparation of standard curves, sample preparation and analysis of a sample with unknown content of a known substance, data interpretation, evaluation and logging.

Experimental design and analysis I | Lecture (VO) | Coursecode: 220574112

Design of experiments

1.50

ECTS

1.00

SWS

Statistical planning of experiments in pre-clinical and analytical- technical contexts, experimental strategies and creating experimental designs (screening designs, full factorial designs, response surface designs, etc.), evaluation of experimental data with analysis of variance and /or regression, critical success factors in DoE.

Experimental design and analysis I | Lecture/Practical (IL) | Coursecode: 220574111

Quality assurance and preanalytics

2.50

ECTS

2.00

SWS

Need for quality standards by the Food and Drug Administration (FDA) and the Environmental Protection Agency (EPA); general criteria for operating test laboratories (ÖNORM EN 45001, ISO/IEC 17025); definition, explanation and commenting of quality assurance terminology, controls for quality assurance, preanalytical steps and relevance for results of analysis, preanalytical artefacts and applications

Experimental design and analysis I | Lecture (VO) | Coursecode: 220574110

Statistical procedures, data preparation and data analysis

3.00

ECTS

2.00

SWS

Application-oriented statistical foundations, descriptive statistics for the biometrics laboratory, tools and instruments for statistical data analysis and interpretation, quality assurance based on statistical procedures.

General chemistry skills | Lecture (VO) | Coursecode: 220574103

General chemistry and stoichiometry

3.00

ECTS

2.00

SWS

General and phyisical chemistry (foundations of the atomic theory and chemical bindings, the law of mass action and the chemical equilibrium, chemical reaction types (acid-base reactions, radical reactions, redox reactions), terminology of chemical compounds, chemical and enzymatic catalysis, Gibbs Function and relevance for chemical reactions, kinetics of chemical reactions, laws of thermodynamics) Stoichiometry (forming and parameterizing of chemical reaction equations, calculations based on chemical reactions and serial dilutions, calculating aqueous mixtures, equilibrium constants and acid-base reactions, pH definition, pKS and pKB and buffer systems, concentration units and absolut amounts)

General chemistry skills | Lecture (VO) | Coursecode: 220574105

Organic chemistry

4.50

ECTS

3.00

SWS

Chemical bonding in organic molecules, structure of organic biomolecules, Stereochemistry, Classification and properties of substance classes, Important functional groups and their reactions, Important reaction mechanisms of organic chemistry relevant for subsequent courses

Molecular biology and analysis | Lecture (VO) | Coursecode: 220574104

Introduction to molecular biology

2.00

ECTS

1.00

SWS

genes, gene structure, chromosomes, information flow from gene to protein, DNA replication, transcription and translation, genetic code, tools of molecular biology (bacteria, viruses, enzymes) synthetic oligonucleotides. Standard methods in molecular biology

Molecular biology and analysis | Lecture/Practical (IL) | Coursecode: 220574109

Molecular analysis I

2.00

ECTS

2.00

SWS

Applied molecular analysis including electrophoretic separations of nucleic acids and proteins

Molecular biology and analysis | Lecture (VO) | Coursecode: 220574108

Molecular cell biology and genetics

4.50

ECTS

3.00

SWS

Advanced genetics and cell biology of model organisms, Applications for genetic analysis

Chromatographic and mass spectrometric applications II | Practical (UE) | Coursecode: 220574202

Chromatographic and spectrometric techniques II

3.00

ECTS

3.00

SWS

Practical application of LC and/or GC-based separation methods with intermediate complexity in respect to sample preparation, separation and detector integration/coupling: – Application structure-spedific sample preparation techniques for isolation of a panel of substances within complex sample matrices and subsequent preparation, enrichment or chemical derivatization – chromatographic method optimization based on specific key parameters for application of multi methods e.g. in trace analytics – Application of appropriate measures for method optimization in respect to separation power and efficient ionization of target analyt – Integraton of univeral and selective detectors for application of the aquired knowledge in the fields of spectrometric methods / mass spectrometry to qualitative problems in respect to substance identification – Practice with spectral databases and independent design of an data analysis method

Chromatographic and mass spectrometric applications II | Lecture (VO) | Coursecode: 220574201

Molecular and mass spectrometry

4.00

ECTS

3.00

SWS

UV/VIS spectrometry – principles of UV/VIS spectroscopy – principle structure and function of a UV/VIS spectrometer – chromophores and auxochromes – spectroscopical phenomena of chemical compounds – structural analysis Infrared and Raman spectrometry – principles of IR spectroscopy – principle structure and function of an IR and Raman spectrometer – characteristic vibrational spectra of chemical compounds – structural analysis Mass spectrometry – principles of mass spectrometry – principle structure and function of a mass spectrometer – techniques of ionisation of molecules and source design – main fragmentation reaction of organic compounds – performance of different technologies and their scope of application

Chromatographic and mass spectrometric applications II | Seminar (SE) | Coursecode: 220574203

Spectrum Interpretation

2.00

ECTS

2.00

SWS

– model procedures for interpretation of spectra of chemical compounds – interpretation and discussion of UV/VIS spectra with practical examples – interpretation and discussion of IR spectra with practical examples – theory of isotope distribution in mass spectrometry – advanced molecule-ion theory in mass spectrometry – priciple mechanisms of ion fragmentation in GC-MS ionization technique – priciple mechanisms of ion fragmentation in LC-MS ionization technique – interpretation and discussion of MS spectra with practical examples – postulation of molecular structure with the combined interpretation of UV/VIS, IR and MS spectra

Experimental Design and Analysis II | Seminar (SE) | Coursecode: 220574208

Medical and scientific ethics

2.00

ECTS

1.00

SWS

Ethics in biomedicine (in the fields of gene technology, molecular diagnostics, gene therapy, planning of experimental and clinical studies, stem cells, personalized medicine), ethics committee, patient solicitors, ethics in transplantation, research, reproductive medicine, intensive care , palliative care, socioeconomical aspects, animal experiments

Experimental Design and Analysis II | Lecture/Practical (IL) | Coursecode: 220574209

Validation of analytical methods

2.00

ECTS

2.00

SWS

Prerequisites for the validation of an analytical method, validation parameters and their examination (reproducibility, repeatability, selectivity, sensitivity, recovery, precision, accuracy, detection and quantification limits, robustness), methods to characterize analytical methods, flow chart for method validation, release of method, documentation.

Molecular and diagnostic applications | Lecture/Practical (IL) | Coursecode: 220574207

Molecular Diagnostics I

4.00

ECTS

2.00

SWS

Principles and advanced knowledge of molecular diagnostics, diagnostic strategies and procedures, carrier and test systems, biomarkers, criteria for method selection, practical examples in medicine, industry and environment ( detection of genetic diseases, forensic medicine, detection of genetically modified organsims, detection of pathogens in body fluids, drinking water, animal feeds and food); methods with a focus on their application in diagnostics: immunoassays (ELISA, immuno PCR, Westernblot), PCR (real time PCR, digital PCR), mutation analysis (SNP genotyping, DNA fingerprinting), microarray based methods (CGH arrays, SNP arrays, protein and antibody arrays), quality assurance

Molecular and diagnostic applications | Lecture/Practical (IL) | Coursecode: 220574206

Molecular and genetic engineering methods

5.00

ECTS

3.00

SWS

Design, apply and interpretation of selected molecular and gene technology methods including isolation of nucleic acid (e.g. from complex sample matrices like soil samples, waste water, breath and plasma), amplification and detection of nucleic acids by using different detection formats (e.g. digital PCR, qPCR, Sequenziertechnologien); Gene technology methods (vector and host systems, CRISPR-Cas9 based genome editing, mutagenesis, protein expression) and examination and influencing of gene regulation (real-time PCR, RNA interference,…) and high-throughput technologies criteria for choice of method; selection of the most suitable method for various applications (e.g. forensic science, waste water monitoring, antibody production).

OMICs technologies and bioinformatics | Lecture/Practical (IL) | Coursecode: 220574204

Applied OMICS technologies

4.00

ECTS

3.00

SWS

Background and special considerations of Transcriptomics/(epi-) genomics/proteomics/glycomics/lipidomics/metabolomics: Biological Question, Experimental Design, Experiment, Image Analysis, Normalization, Data Mining, Verification and Interpretation; on the basis of applied tasks

OMICs technologies and bioinformatics | Lecture (VO) | Coursecode: 220574205

Applied bioinformatics

4.00

ECTS

2.00

SWS

Introduction and use of different data bases (sequence, structure, genome and interaction data bases), prominent web portals (NCBI, ExPASy, KEGG), principles of sequence analysis (alignment, assembly, annotation), homologies, prediction programs (splice sites, promoter, mutations), simulation techniques, simulation of biological processes and systems, modelling of cellular systems.

Data interpretation and dissemination | Lecture/Practical (IL) | Coursecode: 220574302

Data management, data interpretation and structure analysis

4.00

ECTS

2.00

SWS

Biological data management and knowledge management systems, data base structure and data formats, procedures of exploratory data analysis, normalization procedures and data reduction, data and structure analysis with multivariate statistical approaches (e.g. principal component analysis (PCA), independent component analysis (ICA), PLS discriminant analysis (PLS-DA), cluster analysis.

Data interpretation and dissemination | Seminar (SE) | Coursecode: 220574301

Scientific Writing and Dissemination

6.00

ECTS

3.00

SWS

Tips and tricks for compiling written research papers (structure, referencing, graphical representation), professional abstract writing, short papers, full papers, posters and presentations in English language and planing of interdisciplinary grant proposals

Mass spectrometry | Lecture (VO) | Coursecode: 220574305

Advanced chromatographic separation techniques

3.00

ECTS

2.00

SWS

Detailed mechanisms of gradient separation and isocratic separation, Special chromatographic extraction and enrichment, techniques (solid-phase extraction (SPE), in-tube extraction (ITEX), liquid-liquid extraction (LLE)) Special column materials (carrier materials and ligands) and their application, Mobile phases (mixtures and viscosity), Nano LC, Detailed discussion of applications in chromatography

Mass spectrometry | Lecture/Practical (IL) | Coursecode: 220574304

Special analytical methods

13.00

ECTS

7.00

SWS

Design of complex analysis workflows from selection of separation and detection method, preparation of necessary materials and standards including design of an analysis plan that considers the following aspects: – Sample stabilisation and storage – Enrichment / Isolation of analytes from complex matrices – Optimization of sample preparation (solvent selection, optimization of pH and ion strength of solvent) – Optimization strategies for eluents and stationary phases relevant for chromatographic separation – Demand-oriented optimization of instrument parameters for robust and sensitive detection of analytes – Calibration of required analysis systems – Chemometric methods for optimization of chromatographic processes – Demonstration of multivariate analysis and biostatistics during data analysis – Systematic error management

Mass spectrometry | Seminar (SE) | Coursecode: 220574303

Special chapters of mass spectrometry

4.00

ECTS

3.00

SWS

Theoretical background of mass analyzers Combination of ionizing techniques and mass analyzers Application of common MS systems High-resolution mass spectrometry Database supported identification of spectra Identification of unknown samples in MS Mass spectra of different classes of compounds Detailed mechanisms of ion fragmentation Potential and challenges of data processing Detailed sample preparation Potential of derivatization techniques MALDI-MS and SIMS-MS imaging Development of solution strategies with MS for problems in biology

Molecular diagnostics, therapy and production | Lecture (VO) | Coursecode: 220574307

Gene therapy

2.00

ECTS

1.50

SWS

Principles, methods and vectors in somatic gene therapy, advantages and disadvantages of the individual administration form, risks, legal aspects, other innovative therapy strategies, application examples

Molecular diagnostics, therapy and production | Lecture/Practical (IL) | Coursecode: 220574306

Molecular diagnostics 2

5.00

ECTS

3.00

SWS

Criteria for the choice of biomarkers; procedures to identify new biomarkers (medical and environmental); single cell analysis; special knowledge in next generation sequencing, practical application and data evaluation.

Molecular diagnostics, therapy and production | Lecture (VO) | Coursecode: 220574309

Personalized medicine

3.00

ECTS

2.00

SWS

Methods and specific aspects of personalized medicine including analytical challenges, possibilities and risks of individualized medicine.

Molecular diagnostics, therapy and production | Lecture/Practical (IL) | Coursecode: 220574310

Special interdisciplinary applications

5.00

ECTS

2.50

SWS

Interdisciplinary applications in the field of molecular techniques incl. Diagnostic methods e.g. based on nanotechnology

Molecular diagnostics, therapy and production | Lecture/Practical (IL) | Coursecode: 220574308

Tissue and Protein Engineering

5.00

ECTS

3.00

SWS

Stem cells: Definition of different categories, Origin and Generation of stem cells, Growth, Differentiation/Development, Niches, manipulation/modification and characterization; Tissue engineering: Organisation and structure of tissue, immune tolerance, regeneration, wound healing, biomaterials and extracellular scaffolds; Culture conditions (2/3D-Models, bio-reactors, biomechanical stimulation), Applications (2/3D-Models, stemcell therapies, Tissue Eng.), Research project focused on regenerative medicine: Selection of novel therapeutic solutions for clinical problems, strategies for repair, replacement and regeneration of different tissues and organs; isolation of stem cells from organs and reprogramming, Setup of 3D- models (Organoids, Organ-on-a-chip,…), toxicity assays. Protein Engineering: Possibilities of targeted protein structure changes (focusing on protein design, synthetic biology, structural analysis, mutagenesis, demonstration of protein design software platforms), …

Master thesis | Modul/Final Examination (FA) | Coursecode: 220574402

Master exam

2.00

ECTS

0.00

SWS

Presentation and defence of master thesis, Exam in relation to study plan relevant contents

Master thesis | Master's Thesis (MA) | Coursecode: 220574401

Master thesis

26.00

ECTS

0.50

SWS

The aim of the master thesis is to define and examine an innovative problem using scientific methods with high level of independence, and to document and discuss the results in a thesis.

Master thesis | Seminar (SE) | Coursecode: 220574403

Master thesis seminar

2.00

ECTS

1.00

SWS

This seminar accompanies the writing process of the master thesis and provides guidelines for writing a master thesis. In addition, the students are offered professional and methodological support.

Bioinformatics and statistical procedures | Lecture/Practical (IL) | Coursecode: 150754103

Statistical procedures, data preparation and data analysis

4.00

ECTS

2.00

SWS

Application-oriented statistical foundations, descriptive statistics for the biometrics laboratory, tools and instruments for statistical data analysis and interpretation, quality assurance based on statistical procedures.

Biological and chemical foundations | Lecture/Practical (IL) | Coursecode: 150754101

Chemistry and stoichiometry

7.00

ECTS

5.00

SWS

Organic Chemistry: – Stereochemistry – Heterocycles – Structure of biomolecules and their chemical potential – Chemical bonding in organic molecules – Classification and properties of substance classes – Alkanes, alkenes and alkynes – Cycloalkanes and aromatics – Important functional groups and their reactions – Important reaction mechanisms of organic chemistry – Simple chemical synthesis processes Physical Chemistry: – Chemical catalysis – The Gibbs function and its role in chemical reactions – Kinetics of chemical reactions – Theory of chemical bonding – The laws of thermodynamics Stoichiometry: – Calculation of aqueous mixtures – Calculation of complex kinetic processes – Calculation of enzyme-catalyzed reactions – Working with equilibrium constants – Calculation of acid / base reactions – The pH concept and calculation of buffer systems – Calculate of redox reactions – Calculation of basic kinetic processes

Biological and chemical foundations | Lecture (VO) | Coursecode: 150754108

Introdcution into molecular biology (for bachelor graduates with a chemical/technical focus; synchronising molecular biology)

2.00

ECTS

2.00

SWS

Structure of prokaryotes and eukaryotes, structure and function of macromolecules (proteins, DNA, RNA, carbohydrates), genes, gene structure, chromosomes, information flow from gene to protein, DNA replication, transcription and translation, genetic codes, tools of molecular biology (bacteria, viruses, enzymes) vectors, synthetic oligonucleotides. Standard methods in molecular biology: Extraction of nucleic acids, determination of concentrations, detection of nucleic acids (blotting procedure), electrophoretic separation (nucleic acids and proteins), reverse transcription, PCR, blotting procedures, cloning, sequence analysis, foundations and applications of human and cytogenetics (mutations, heredity, diagnostics)

Biological and chemical foundations | Lecture (VO) | Coursecode: 150754107

Introduction into chemistry (for bachelor graduates with a biological focus; synchronising chemistry)

2.00

ECTS

2.00

SWS

General chemistry: – basic laws of chemistry – foundations of the atomic theory and chemical bindings – the periodic table of elements – the law of mass action and the chemical equilibrium – types of chemical reaction (acid-base reactions, radical reactions, redox reactions) – terminology of chemical bindings Stoichiometry: – forming and paramterizing of chemical reaction equations – calculating chemical reactions – calculating serial dilutions – calculating aqueous mixtures

Biological and chemical foundations | Lecture/Practical (IL) | Coursecode: 150754104

Molecular cell biology, genetics and foundations of gene technology

7.00

ECTS

5.00

SWS

Legal bases of genetic engineering, differences in bacterial and mammalian genetics, possibilities of gene transfer in prokaryotes and eukaryotes, selection, protein expression systems, cell lysis/cell disruption, genetic regulatory mechanisms, cellular transport systems, protein degradation, cell-cell communication, mutagenesis, classical genetics and advanced genetics by the example of selected host organisms, basics of microbiology and virology, micro-organisms and viruses as tools of genetic engineering, species definition/taxonomy, laboratory animal science, analysis and cross-breeding of mouse lines, the production of transgenic organisms, special features of stem cells, discussion about the above-mentioned contents in specific fields of application

Chromatographical and mass spectrometry techniques | Lecture/Practical (IL) | Coursecode: 150754102

Chromatographic and electrophoretic separation techniques I

4.00

ECTS

3.00

SWS

Foundations of chromatographic techniques: – Taxonomy of chromatographic procedures – principles of chromatographic separation – liquid chromatography – instrumentation – important parameters – Van Deemter equation – stationary phases – mobile phases – reversed-phase chromatography – normal-Phase chromatography – ion exchange chromatography – hydrophilic interaction chromatography – chiral chromatography – affinity chromatography – gel-permeation chromatography – principles of isocratic separation and gradient separation – detectors – Gas chromatography – instrumentation – important parameters – Raoult’s law – separation columns – carrier gases – retention mechanisms – detectors – principles of temperature dependent separation of volatile and semi-volatile compounds Foundations of electrophoretical techniques: – principles of electrophoretical separation – capillary zone electrophoresis – instrumentation – retention mechanisms …

Scientific methods and research techniques | Seminar (SE) | Coursecode: 150754106

Data bases and effective literature search

2.00

ECTS

1.00

SWS

Search strategies for literature and other databases (introduction to relevant databases, selection of search keys, systematic approach, primary sources, scientific relevance, search documentation ); practical approach models for efficient and effective literature management, reference management software.

Scientific methods and research techniques | Seminar (SE) | Coursecode: 150754105

Team management & social media

2.00

ECTS

1.00

SWS

Development of attitudes and strategies that aid in increasing team performance and individual satisfaction and reduce conflict when collaborating in a team. The students get to know how to use digital media when working in a team and how to use professionally digital resources of the UAS JOANNEUM (audio/video conferencing, resource sharing, the LMS moodle, VPN access to resources of the university intranet), data protection and legal issues ( UAS JOANNEUM data protection guide).

Bioinformatics and statistical procedures | Lecture/Practical (IL) | Coursecode: 150754209

Applied bioinformatics

4.00

ECTS

2.00

SWS

Introduction and use of different data bases (sequence, structure, genome and interaction data bases), prominent web portals (NCBI, ExPASy, KEGG), principles of sequence analysis (alignment, assembly, annotation), homologies, prediction programs (splice sites, promoter, mutations), system theory and systems thinking, simulation techniques, simulation of biological processes and systems, modelling of cellular systems.

Bioinformatics and statistical procedures | Lecture/Practical (IL) | Coursecode: 150754207

Quality assurance and validation in analytics

2.00

ECTS

2.00

SWS

Need for quality standards by the Food and Drug Administration (FDA) and the Environmental Protection Agency (EPA); general criteria for operating test laboratories (ÖNORM EN 45001, ISO/IEC 17025); definition, explanation and commenting of quality assurance terminology, prerequisites for the validation of an analytical method, validation parameters and their examination (reproducibility, repeatability, selectivity, sensitivity, recovery, precision, accuracy, detection and quantification limits, robustness), methods to characterize analytical methods, flow chart for method validation, release of method, documentation.

Chromatographical and mass spectrometry techniques | Practical (UE) | Coursecode: 150754203

Chromatographical and spectrometrical techniques

4.00

ECTS

3.00

SWS

putting theory into practice: instrumental main component and trace analytics: – creation of an analysis plan – production of standard solutions and serial dilutions – measuring of standard samples with chromatographical and spectrometrical techniques – creation of standard curves – analysis of a sample containing an unknown amount of a well-known analyte – sample collection – sample preparation – separation of complex mixtures with LC and GC – interpretation of the data – evaluation and logging In addition to handling of single procedures the focus is placed on the holistic aspect of the analytical procedure

Chromatographical and mass spectrometry techniques | Seminar (SE) | Coursecode: 150754208

Interpretation of spectra

2.00

ECTS

2.00

SWS

– model procedures for interpretation of spetra of chemical compounds – interpretation and discussion of UV/VIS spectra with practical examples – interpretation and discussion of IR spectra with practical examples – theory of isotope distribution in mass spectrometry – advanced molecule-ion theory in mass spectrometry – priciple mechanisms of ion fragmentation in GC-MS ionization technique – priciple mechanisms of ion fragmentation in LC-MS ionization technique – interpretation and discussion of MS spectra with practical examples – postulation of molecular structure with the combined innterpretation of UV/VIS, IR and MS spectra

Chromatographical and mass spectrometry techniques | Lecture (VO) | Coursecode: 150754202

Molecular and mass spectrometry

3.00

ECTS

3.00

SWS

UV/VIS spectrometry – principles of UV/VIS spectroscopy – principle structure and function of a UV/VIS spectrometer – chromophores and auxochromes – spectroscopical phenomena of chemical compounds – structural analysis Infrared and Raman spectrometry – principles of IR spectroscopy – principle structure and function of an IR and Raman spectrometer – characteristic vibrational spectra of chemical compounds – structural analysis Mass spectrometry – principles of mass spectrometry – principle structure and function of a mass spectrometer – techniques of ionisation of molecules and source design – main fragmentation reaction of organic compounds – performance of different technologies and their scope of application

Molecular techniques and life sciences | Lecture/Practical (IL) | Coursecode: 150754204

Applied OMICS technologies (transcriptomics, genomics, proteomics, metabolomics)

4.00

ECTS

3.00

SWS

Transcriptomics/(epi-) genomics/proteomics/glycomics/lipidomics/metabolomics: Biological Question, Experimental Design, Experiment, Image Analysis, Normalization, Data Mining, Verification and Interpretation; on the basis of concrete tasks

Molecular techniques and life sciences | Lecture/Practical (IL) | Coursecode: 150754206

Medical and environmental molecular diagnostics 1

4.00

ECTS

2.00

SWS

Principles and advanced knowledge of molecular diagnostics, diagnostic strategies and procedures, carrier and test systems, biomarkers, criteria for method selection, practical examples in medicine, industry and environment ( detection of genetic diseases, forensic medicine, detection of genetically modified organsims, detection of pathogens in body fluids, drinking water, animal feeds and food); methods with a focus on their application in diagnostics: immunoassays (ELISA, immuno PCR, Westernblot), PCR (real time PCR, digital PCR), mutation analysis (SNP genotyping, DNA fingerprinting), microarray based methods (CGH arrays, SNP arrays, protein and antibody arrays), quality assurance

Molecular techniques and life sciences | Lecture/Practical (IL) | Coursecode: 150754201

Methods of molecular biology and gene technology

5.00

ECTS

3.00

SWS

Isolation of nucleic acid (e.g. from complex sample matrices like soil samples, waste water, breath and plasma), amplification and detection of nucleic acids by using different detection formats (e.g. digital PCR, qPCR, in situ…) selection of suitable vector and host systems, examination and influencing of gene regulation (real-time PCR, RNA interference,…) mutagenesis, high-throughput technologies (generation of libraries, next generation sequencing, RNAseq, micro-arrays (oligonucleotide, peptide arrays), ChIP-Seq, methylation analysis), protein expression systems, in situ detection of proteins, principles of protein design; criteria for choice of method; selection of the most suitable method for a concrete question (e.g. forensic science, waste water monitoring, antibody production).

Molecular techniques and life sciences | Lecture (VO) | Coursecode: 150754205

Nanotechnology

2.00

ECTS

1.00

SWS

Nanotechnology in medicine, medical technology and environment: molecular targeting, molecular imaging, drug delivery, surface coating, nanotoxicology, environmental monitoring

Bioinformatics and statistical procedures | Lecture/Practical (IL) | Coursecode: 150754304

Data management, data interpretation and structure analysis

4.00

ECTS

2.00

SWS

Biological data management and knowledge management systems, data base structure and data formats, procedures of exploratory data analysis, normalization procedures and data reduction, data and structure analysis with multivariate statistical approaches (principal component analysis (PCA), independent component analysis (ICA), PLS discriminant analysis (PLS-DA), cluster analysis.

Scientific methods and research techniques | Lecture (VO) | Coursecode: 150754301

Design of experiments

2.00

ECTS

1.00

SWS

General and statistical planning of experiments in (pre-) clinical and analytical- technical contexts, experimental strategies and creating experimental designs (2k – fractional factorial designs, full factorial designs, response surface designs), evaluation of experimental data with analysis of variance and /or regression, critical success factors in DOE.

Scientific methods and research techniques | Lecture/Practical (IL) | Coursecode: 150754303

Interdisciplinary research project

4.00

ECTS

2.00

SWS

Implementation of an independent project (in team work) with a clear initial research question, information and literature search, designing and conducting appropriate experiments, data evaluation and interpretation of the results, write-up and presentation of the results.

Scientific methods and research techniques | Seminar (SE) | Coursecode: 150754302

Scientific writing and presentation

2.00

ECTS

1.00

SWS

Tips and tricks for compiling written research papers (structure, referencing, graphical representation), professional abstract writing, short papers, full papers, posters and presentations in English language.

Mass spectrometry | Lecture (VO) | Coursecode: 150754307

Chromatographic separation techniques II

2.00

ECTS

2.00

SWS

Detailed mechanisms of gradient separation and isocratic separation Special chromatographic extraction and enrichment techniques (solid-phase extraction (SPE), in-tube extraction (ITEX), liquid-liquid extraction (LLE)) Special column materials (carrier materials and ligands) and their application Mobile phases (mixtures and viscosity) Nano LC Injection techniques in gas chromatography Detailed discussion of applications in chromatography

Mass spectrometry | Practical (UE) | Coursecode: 150754309

Focus-specific project work

6.00

ECTS

1.00

SWS

The students work out representative analytical methods according to the given requirements. They cover the complete process from formulating the research question, searching for relevant literature and resources, preparing the samples to carrying out the analytical procedure and evaluating and disseminating the results. Case studies enhance their awareness of the complexity of bioanalytical problems. Thus, the students are enabled to draft independently application oriented solution possibilities to complex analytical problems. In this course the students need to compile and present their own project papers and must answer questions about the analytic process that they have dealt with.

Mass spectrometry | Lecture/Practical (IL) | Coursecode: 150754308

Method development and optimization

2.00

ECTS

2.00

SWS

Techniques of sample collection Stabilizing and storage of samples Concentration and isolation of analytes from complex matrices Optimization of sample preparation (choice of suitable solvents, optimizing of pH value and ion strength of a solvent) Optimization strategies for eluents and stationary phases Selectivity of columns in chromatography Optimization of reversed phase chromatography Optimization of normal phase chromatography Using principal component analysis (PCA) to evaluate chromatographical procedures Chemometrical methods for optimizing the chromatographic process Optimization techniques in gas chromatography Systematic search for errors in LC-MS and GC-MS

Mass spectrometry | Practical (UE) | Coursecode: 150754306

Practical exercises in mass spectrometry

4.00

ECTS

4.00

SWS

Planning of complex analysis processes and choosing the appropriate LC/MS and GC/MS techniques Preparation of tools and standard samples and calibration of the required analysis systems Optimization of parameters of the analytic devices that meet the requirements for safe and sensitive detection of targeted analytes Experimental identification of limit of detection and limit of quantitation of the analytes Separation of complex substance mixtures using computer-assisted mass spectrometry techniques Identification of characteristic ions for qualitative and quantitative measurement of analytes Application of appropriate procedures for method optimization with regard to separation and effective ionization of target analytes Computer-aided data processing and reporting

Mass spectrometry | Seminar (SE) | Coursecode: 150754305

Special chapters of mass spectrometry

4.00

ECTS

3.00

SWS

Theoretical background of mass analyzers Combination of ionizing techniques and mass analyzers Application of common MS systems High-resolution mass spectrometry Database supported identification of spectra Identification of unknown samples in MS Mass spectra of different classes of compounds Detailed mechanisms of ion fragmentation Potential and challenges of data processing Detailed sample preparation Potential of derivatization techniques MALDI-MS and SIMS-MS imaging Development of solution strategies with MS for problems in biology

Molecular diagnostics, therapy and production | Lecture (VO) | Coursecode: 150754314

Ethics in biomedicine

1.00

ECTS

0.50

SWS

Ethics in biomedicine (in the fields of gene technology, molecular diagnostics, gene therapy, planning of experimental and clinical studies, stem cells, personalized medicine)

Molecular diagnostics, therapy and production | Lecture/Practical (IL) | Coursecode: 150754311

Gene therapy

3.00

ECTS

2.00

SWS

Principles, methods and vectors in somatic gene therapy, advantages and disadvantages of the individual administration form, risks, legal aspects, other innovative therapy strategies like cloning or stem cell therapy, application examples

Molecular diagnostics, therapy and production | Lecture/Practical (IL) | Coursecode: 150754315

Interdisciplinary applications

4.00

ECTS

2.00

SWS

functional genomics; multidsiciplinary networking and interdisciplinary practical examples of molecular techniques

Molecular diagnostics, therapy and production | Lecture/Practical (IL) | Coursecode: 150754310

Medical and environmental molecular diagnostics 2

4.00

ECTS

3.00

SWS

Criteria for the choice of biomarkers; procedures to identify new biomarkers (medical and environmental); single cell analysis; special knowledge in next generation sequencing, practical application and data evaluation.

Molecular diagnostics, therapy and production | Lecture (VO) | Coursecode: 150754313

Pharmacogenomics and personalized medicine

2.00

ECTS

1.50

SWS

Foundations of pharmacogenomics; methods/ background; opportunities and risks of individualized medicine

Molecular diagnostics, therapy and production | Lecture/Practical (IL) | Coursecode: 150754312

Protein design and engineering

4.00

ECTS

3.00

SWS

In silico modelling, possibilities of structure change (mutagenseis, synthetic biology, tags, fusion proteins) production systems, practical examples from vaccine development, dietary supplements, medication.

Master thesis | Master's Thesis (MA) | Coursecode: 150754401

Master thesis

26.00

ECTS

0.00

SWS

The aim of the master thesis is to work out independently an innovative job-specific topic, to examine it scientifically and to record the results in a thesis.

Master thesis | Seminar (SE) | Coursecode: 150754401

Master thesis seminar

4.00

ECTS

2.00

SWS

The aim of the master thesis is to work out independently an innovative job-specific topic, to examine it scientifically and to record the results in a thesis.